《植物生理学报》 2021, 57(2): 493-500

AtGSTZ1参与拟南芥盐胁迫响应过程

胡超¹，陈彦成²，张学文¹，赵燕¹，黄丽华^1,*

¹湖南农业大学生物科学技术学院, 长沙410128；²农业农村部长江中下游籼稻遗传育种重点实验室, 湖南省水稻研究所, 长沙410125

通信作者：黄丽华；E-mail: lihua30001@sina.com

摘　要：

酪氨酸降解途径在动物生长发育中发挥着重要作用。然而, 该途径在植物中的功能还需进一步鉴定。马来酰乙酰乙酸异构酶是酪氨酸降解途径中的关键酶。 ζ类谷胱甘肽S-转移酶(GSTZ)具有马来酰乙酰乙酸异构酶活性。为探讨酪氨酸降解途径在植物响应盐胁迫中的作用, 本研究分析了拟南芥(Arabidopsis thaliana) AtGSTZ1基因在盐胁迫下的表达特征以及AtGSTZ1过表达植株对盐胁迫的耐受性, 发现: AtGSTZ1表达受盐胁迫诱导; 在盐胁迫下, AtGSTZ1过表达植株具有较高的种子萌发率和子叶绿化率; 此外, 盐胁迫下过表达植株积累了较少的H₂O₂, 且过氧化氢酶基因CAT1表达量和过氧化氢酶活性都高于野生型。实验结果表明, AtGSTZ1通过调控盐胁迫下H₂O₂的积累而影响拟南芥的耐盐性。本研究结果为证明酪氨酸降解途径在植物响应盐胁迫中的作用提供依据。

关键词：拟南芥; 酪氨酸降解途径; AtGSTZ1; 盐胁迫; H2O2

收稿：2020-09-18   修定：2020-12-21

资助：湖南省教育厅项目(18A100)和农业农村部长江中下游籼稻遗传育种重点实验室(湖南省水稻研究所)开放课题(2018KLMA03)

AtGSTZ1 is involved in salt stress response in Arabidopsis thaliana

HU Chao¹, CHEN Yancheng², ZHANG Xuewen¹, ZHAO Yan¹, HUANG Lihua^1,*

¹College of Bioscience and Biotechnology, Hunan Agricultural University, Changsha 410128, China; ²Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley, Ministry of Agriculture and Rural Affairs, Hunan Rice Research Institute, Changsha 410125, China

Corresponding author: HUANG Lihua; E-mail: lihua30001@sina.com

Abstract:

The tyrosine degradation pathway is crucial for animals, but its role in plants is not well-characterized. Maleylacetoacetate isomerase is a key enzyme in the pathway. The ζ class glutathione S-transferase (GSTZ) has been shown to have activity of maleylacetoacetate isomerase. To understand the function of the tyrosine degradation pathway in plant response to salt stress, AtGSTZ1 expression in Arabidopsis thaliana was analyzed under salt stress. AtGSTZ1 expression was induced by salt stress. AtGSTZ1-overexpression plants were subjected to salt stress. The data show that AtGSTZ1-overexpression plants displayed higher seed germination rates and cotyledon greening rates compare to wild-type plants under salt stress. In addition, AtGSTZ1 overexpression decreased H₂O₂ accumulation, and elevated expression level of CAT1 and catalase activity in A. thaliana under salt stress. These results indicate that AtGSTZ1 regulates salt tolerance of A. thaliana by regulating H₂O₂ accumulation under salt stress. The data provide further insight into the role of the tyrosine degradation pathway in plant response to salt stress.

Key words: Arabidopsis thaliana; tyrosine degradation pathway; AtGSTZ1; salt stress; H2O2